Fig. 6. Alignment of the downstream regions of the internal transcribed spacer from M. vaginatus and M. steenstrupii. Box A Spacer (bold type) and D4 (red font) are invariant. V3 (bold type) is a stem and loop structure that demonstrates marked sequence variability, especially within M. steenstrupii (compare the terminal loop sequence, indicated by red font). These sequences are contiguous with those in Fig. 5b.

                      Box A Spacer   D4             <-----------------------------------
M. vaginatus                    
SEV2MC1-3F CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCAUCAUCAA------GCAGGUAAGUCUUCUG-------
WD1MC2-5D  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUAUCAUCCG-------
FB1MC1-3C  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUGUCAUCCG-------
FB2MC3-4C  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCGAUCAUCCG-------
FI5MC4-2E  CUCUAGCCA-GAACCUUGAAAACUGCAUAGGAAUUAAGUCA---UCAAA-----GCGUGUUUAUCAUCCG-------
MOA4MC3-3C CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUGUCAUCCG-------
MERMC1-1C  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUAUCAUCCG-------
SEV2MC1-3E CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCUUGCCUAUCGUCGG-------
MERMC1-1B  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUAUCAUCCG-------
CSV2MC1-6B CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUUGAUCAUCCG-------
MOA4MC3-3B CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUGUCAUCCG-------
MOA4MC3-3D CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUGUCAUCCG-------
FB1MC1-3E  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUGUCAUCCG-------
WD1MC2-5C  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUAUCAUCCG-------
FB2MC3-4B  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCUUGUUUAUCGUCCG-------
FI5MC4-2F  CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUUUAUCAUCCG-------
CSV2MC1-6C CUCUAGUCA-GAACCUUGAAAACUGCAUAGAAAUUAAGUCA---UCAAA-----GCGUGUCUAUCAUCCG-------
M. steenstrupii
DG6MC1-3E  GUCCAGUGAAGAACCUUGAAAACUGCAUAGCCAUUAAGU-A---ACAAA----GGCAGGUAAGACCGCUGAUAC--- 
USPCMC1-2D AUCCAGUCA-GCACCUUGAAAACUGCAUAGCAAU-AAGUCA---ACAAA-----GCAGGUAAGAGUAAAGACACU--
MOA1MC1-1F AUCCAGUCA-GCACCUUGAAAACUGCAUAGCAAU-AAGUCA---ACAAA-----GUAGGUAAGAAGAAAGACACU--
MOA2MC2-3B AUCCAGUCA-GCACCUUGAAAACUGCAUAGCAAU-AAGUCA---ACAAA-----GCAGGUAAGACUCAAGACACU--
USPCMC1-2E -UCCAGCGA-GAACCUUGAAAACUGCAUAGCAACAAA----------AAUUCUGUCAGGUA-GAUA--AG-CUGCCC
USPCMC1-2F CUCCAGCGA-GAACCUUGAAAACUGCAUAGCAACAAA----------GAUUCUGUCAGGUA-GAUA---G---CGUU
DG6MC1-3D  CUCCAGCGA-GAACCUUGAAAACUGCAUAGCAACAAA----------AAUUCUGUCAGGUA-GAUA---G---CUUU
MOA2MC2-2A -UCCAGUCA-GAACCUUGAAAACUGCAUAGCAACU-AGUCAGGUAGAAA--CUGUCA-GUA-GUCAGUAGUC-----
MOA1MC1-1D -UCCAGUCA-GAACCUUGAAAACUGCAUAGCGACU-AGUCAGGUAUAAA--CUGUCA-GUA-GUCAGUAGUC-----
MOA1MC1-1E -UCCAGUCA-GAACCUUGAAAACUGCAUAGCAAU-AAGUCA---ACAAA----GU-AGGUAAGAAGAAAGACAC---
	   ----------------V3--------------------------------------------------------> 
M. vaginatus
SEV2MC1-3F ----------A---GAAAUCA-----GGAA-------------------------AUCUUACUGAGC---GACCUC
WD1MC2-5D  ----------A---UUACUCG-----UUCG-------------------------AUAAGAC-AAGC---GACACC  
FB1MC1-3C  ----------AA--GCAAUCG-----UUCG-------------------------GUAUGAC-AAGC---GACACC  
FB2MC3-4C  ----------A---UUGCUCG-----GAGG-------------------------AUGGCAC-AAGC---GACACC 
FI5MC4-2E  ----------A---UUGCUCG-----GAAG-------------------------AUAUGAC-AAGC---GACACC  
MOA4MC3-3C ----------A---UUGCUCG-----GAAG-------------------------AUAUGGC-AAGC---GACACC
MERMC1-1C  ----------A---UUGCUCG-----GAAG-------------------------AUAUGAC-AAGC---GACACC  
SEV2MC1-3E ----------A---UUGCUCG-----UUCG-------------------------AUAGGAC-AAGC---GACACC  
MERMC1-1B  ----------A---UUGCUCG-----GAAG-------------------------AUAUGAC-AAGC---GACACC  
CSV2MC1-6B ----------A---UUGCUCG-----GAAG-------------------------ACAUCAC-AAGC---GACACC  
MOA4MC3-3B ----------AA--GCAAUCG-----UUCG-------------------------AUAUGAC-AAGC---GACACC  
MOA4MC3-3D ----------AA--GCAAUCG-----UUCG-------------------------AUAUGAC-AAGC---GACACC  
FB1MC1-3E  ----------AA--GCAAUCG-----UUCG-------------------------GUAUGAC-AAGC---GACACC  
WD1MC2-5C  ----------A---UUACUCG-----UUCG-------------------------AUAAGAC-AAGC---GACACC  
FB2MC3-4B  ----------AA--GCAAUCG-----UUCG-------------------------GUAUGAC-AAGC---GACACC  
FI5MC4-2F  ----------A---UUGCUCG-----GAAG-------------------------AUAUGAC-AAGC---GACACC  
CSV2MC1-6C ----------A---UUGCUCG-----GGAG-------------------------AUAUGAC-AAGC---GACACC  
M. steenstrupii
DG6MC1-3E  --------------CUAAUAA----------------------------------GUGUCAG-AUUCUUAUAAAGA
USPCMC1-2D --------------C-AAUAA---------------------------------AGUGUCUG-CAUCUUAU-CAGU
MOA1MC1-1F ---------------UAAUAA---------------------------------AGUGUCUG-UAUCUUAU-CAGU
MOA2MC2-3B ---------------UAAUAC---------------------------------AGUGUCUG-CAUCUUAU-CAGG
USPCMC1-2E AAAG-AGAAAAACGCUU-UAAAAA--------------C-GUAAA-G-CUCUUUAGUAGCCA--AUC----ACAGA
USPCMC1-2F AAAG-----------ACAUAAG----------------CUUU--ACGCUA----AGUA-------UC----ACAGA
DG6MC1-3D  AAAG----------CUUAUGG-----------------CUUU-AA-GGUA----AGUA-------UC----ACAGA
MOA2MC2-2A --AGUAG-------GCAAGUCG--CUACAGACAA----CUUUCAA-CCGAC---AGUGG-AU--AUC----ACAGA
MOA1MC1-1D --AGUAG-------GCAAGUCG--CUACAGACAA----CUUUCAA-CCGAC---AGUGGGAU--AUC----ACAGA
MOA1MC1-1E --------------UUAAUA------------AAGUGUCU---------------GUAUCUU--AUC----A--GG